Lab. of Pomology, Graduate School of Agriculture, Kyoto University

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Publications [Presentations 2010-2019]

2019(International)

  • Plant and Animal Genome Conference XXVII (Jan. 12-16th, 2019, San Diego, USA)
    Poster presentation
    Fujikawa*, M., E. Babiker, S. Nishiyama, H. Yamane, K. Shirasawa and R. Tao. Genetic diversity in the southern highbush blueberry cultivars asssessed by ddRAD-Seq.
  • Cherry Section of China Society for Horticultural Science (April 26-27, 2019, Linqu, Shandong, China)
    Invited Keynote Presentation
    Tao*, R. 
    Sweet Cherry Production in Japan: History, Characteristics and Future Perspective
  • Annual Spring Conference of the Korean Society for Horticultural Science (May 22-25, 2019, Gyeongsanbuk-do, Korea)
    Invited Keynote Presentation
    Tao*, R.
    Discovery of the Prunus-specific self-incompatibility recognition system and its horticultural applications
  • ASHS annual conference (Jul. 22-25th, 2019, Las Vegas, USA)
    Poster presentation
    Fujikawa, M., E. Babiker*, S. Nishiyama, H. Yamane, K. Shirasawa and R. Tao. Genetic diversity in the southern highbush blueberry cultivars assessed by ddRAD-Seq.

2018(International)

  • Plant and Animal Genome Conference XXV (Jan. 13-17th, 2018, San Diego, USA)
    Oral presentation
    Akagi*, T., K. Shirasawa, H. Nagasaki, H. Hirakawa, R. Tao, L. Comai and I.M. Henry. The persimmon genome unveils lineage-specific paleoduplication events driving diversification of sexual systems.
    Ono, K., T. Morimoto, T. Akagi, A. Wunsch and R. Tao*. NGS analysis reveals a possible causal mutation conferring self-compatibility in a sweet cherry cultivar Cristobalina.
    Akagi*, T., I.M. Henry, H. Ohtani, T. Morimoto, K. Beppu, I. Kataoka and R. Tao. Identification of the Y-encoded suppressor of feminization in kiwifruit.
    Poster presentation
    Masuda*, K., H. Yamane and R. Tao. Evolutionary analysis of DORMANCY-ASSOCIATED MADS-box genes in Prunus.
    Maeda*, H., T. Akagi and R. Tao. Molecular pathways controlling the "shape" of persimmon fruits.
  • The 25th International Congress on Sexual Plant Reproduction (Jun. 11-16th, 2018, Gifu, Japan)
    Oral presentation
    Akagi*, T., I.M. Henry, H. Ohtani, T. Morimoto, K. Beppu, I. Kataoka and R. Tao. A Y-encoded sex determinant arose via linage-speicific duplication of a cytokinin response regulator in kiwifruit.
    Poster presentation
    Ono*, K., T. Akagi, T. Morimoto, A. Wunsch and R. Tao. Genome re-sequencing of diverse sweet cherry (Prunus avium) individuals reveals a modifier gene mutation conferring pollen-part self-compatibility.
  • The 9th International Rosaceae Genomics Conference (June 26-30th, 2018, Nanjing, China)
    Invited talk
    Tao*, R. New insights into the evolution and mechanisms of the S-RNase-based self-incompatibility in Prunus obtained based on the genome-wide DNA sequencing analysis.
  • Society for Mol. Biol. Evol. (Jul. 8-12th, 2018, Yokohama, Japan)
    Invited talk
    Akagi*, T. Diversification of sexual system: insights from the persimmon genome.
  • The 30th International Horticulture Congress (Aug. 12-16th, 2018, Istanbul, Turkey)
    Keynote speaker
    Tao*, R. New insights into the distinct S-RNase-based self-incompatibility mechanisms in Prunus
    Oral presentation
    Yamane*, H., Y. Kitamura, T. Habu, Wenxing Chen and R. Tao. Identification of QTLs for chilling and heat requirements for bud dormancy release in Prunus mume and their co-localization with the DAM6 eQTL
    Osako*, Y., H. Yamane, S.Y. Lin, P-A. Chen, M. Gao-Takai and R. Tao. Effects of cytokinin on female flower formation in litchi.
    Ono*, K., T. Morimoto, T. Akagi, A. Wunsch and R. Tao. Characterization of the pollen-part modifier gene involved in self-incompatibility reaction in sweet cherry
    Sonoda*, M., T. Akagi, S.S. Sugano, K. Beppu, I. Kataoka and R. Tao. Mechanism of male organ differentiation mechanism in the sex determination system of kiwifruit.
    Poster presentation
    Yamane*, H., K-T. Li, R. Matsuzaki, S. Ebihara, T-S. Li, M. Omori and R. Tao. Comparative observations of phenological characters of continuously flowering high-bush blueberry 'Blue Muffin' in temperate and subtropical climates.
  • HZAU-NTU-KU Joint Horticulture Symposium (Sep. 3-6th, 2018, Wuhan, China)
    Oral presentation
    Tao*, R. Distinct evolution and mechanisms of the S-RNase-based self-incompatibility in Prunus as indicated from the recent genome-wide DNA sequencing analyses
    Yamane*, H.  Genetic and molecular regulation of bud dormancy in Prunus mume
    Akagi*, T. Diversification of sexual system: insights from the persimmon genome
    Nishiyama*, S. Genetics of proanthocyanidins accumulation control in persimmon fruit
    Poster presentation
    Chen*, W. ChIP-Seq analysis for understanding the chilling-mediated histone modification during bud dormancy and bud break in apple
    Chang*, E-T. Characterization of MGST, the pollen modifier gene of the gametophytic self-incompatibility in sweet cherry
    Hirakawa*, M. Studies on the establishment of in vitro pollen tube growth test system for self-incompatibility studies in Prunus
    Maeda*, H. Gene networks underlying the diversity in persimmon fruit shapes
    Makimoto*, R. Studies on the Agrobacterium-mediated transformation of Diospyros lotus and D. oleifera
    Obata*, K. Transformation to induce precocious flowering in several Diospyros spp.
    Li*, T. Effects of pre-harvest UV-B light on fruit development, flavonoid metabolism and postharvest biological characteristics in blueberry
    Ebihara*, S. Effects of pollen parent on fruit quality of highbush blueberry
    Omori*, M. Promotion of flowering by CRISPR/Cas9-mediated genome editing in blueberry
    Sonoda*, M. Mechanism of male organ differentiation mechanism in the sex determination system of kiwifruit
    Shimao*, S. Mechanism underlying expression of hermaphroditism in Actinidia callosa
  • The 6th Plant Dormancy Symposium (Oct. 23-26th, 2018, Kyoto, Japan)
    Oral presentation
    Chen*, W., H. Yamane, Y. Tamada, M. Matsushita, Z. Luo and R. Tao. Epigenome analysis reveals the role of histone modifications in the chilling-regulated gene expression in apple dormant buds
    Yamane,*, H. Genetic and molecular regulation of bud dormancy in Prunus mume
    Poster presentation
    Li*, T., H. Yamane, M. Tomiyama and R. Tao. Characterization of continuous flowering blueberry 'Blue Muffin' and the distinctive effect of hydrogen cyanamide on flowering.
    Matsushita*, M., H. Yamane, S. Nishiyama, C. Honda, K. Okada, S. Moriya and R. Tao. Functional characterization of apple FLC-like gene in winter dormant buds

2017(International)

  • Plant and Animal Genome Conference XXV (Jan. 14-18th, 2017, San Diego, USA)
    Oral presentation
    Nishiyama*, S., N. Onoue, A. Kono, A. Sato, K. Yonemori and R. Tao. Toward isolation of a gene conferring astringency loss in persimmon fruit.
    Akagi, T., I. M. Henry, T. Kawai, L. Comai and R. Tao*. Sex determination in oriental persimmon (Diospyros kaki).
  • ISHS 8th cherry symposium (Jun. 5-9th, 2017, Yamagata, Japan)
    Keynote presentation
    Tao*, R. Recent advances in our understanding of the S-RNase-based gametophytic self-incompatibility system in Prunus.
    Oral presentation
    Morimoto*, T., T. Akagi and R. Tao. Insights into the evolution and establishment of the Prunus-specific self-incompatibility recognition mechanism.
    Ono*, K., T. Morimoto, T. Akagi, Ana Wunsch and R. Tao. Whole genome sequencing approach to identify pollen-part modifier conferring self-compatibility in sweet cherry 'Clistobalina'. 
    Poster presentation (*ISHS student presentation awardee)
    Masuda*, K., H. Yamane, K. Ikeda, T. Tetsumura, M. Gao-Takai and R. Tao. Effects of chilling accumulation on DORMANCY-ASSOCIATED MADS-box gene expressions in sweet cherry 'Satonishiki'.
  • Kyoto Univ. & National Taiwan Univ. Workshop (Mei-Fong Forum 2017) (Jun. 29-Jul. 1, 2017, MeiFeng, Taiwan)
    Oral Presentation
    Nishiyama*, S. Genetic analysis of the non-astringent trait in persimmon fruit
    Kuramoto*, A. Studies on the tannin accumulation in persimmon fruit
    Yang*, H. Molecular pathway to determine flower sexuality in oriental persimmon
    Osako*, Y. S. Lin, H. Yamane, P. Chen and R. Tao. Effect of CPPU on flowering of litchi (Litchi chinensis)
    Matsuzaki*, R. Effects of pollination on fruit development of 'Blue Muffin', a blueberry showing continuous flowering in field condition
    Ono*, K. Whole genome sequencing approach to identify pollen-part modifier conferring self-compatibility in sweet cherry 'Cristobalina'
    Matsushita*, M. Histone modification in apple FLC-like gene during dormancy release
    Poster Presentation
    Ebihara*, S. Characterization of functional components in 'Blue Muffin', a continuously flowering blueberry cultivar
    Maeda*, H. Molecular mechanisms controlling "shape" of persimmon fruits
    Makimoto*, R. Studies on the production of transgenic Diospyros lotus and D. oleifera plants with the male determinant gene, OGI, in Diospyros genus
    Masuda*, K. Effects of chilling accumulation on DORMANCY-ASSOCIATED MADS-box gene expressions in sweet cherry 'Satonishiki'
    Sonoda*, M. Male organ differentiation mechanism in the sex determination system of kiwifruit
    Tam*, C. Identification of genomic locus determining the size difference between Totsutanenashi persimmon and its revertant
    Fujikawa*, M. QTL analysis of Diospyros lotus, a diploid model species of polyploid D. kaki
    Hirakawa*, M. Development of the in vitro pollen tube growth test to reproduce self-incompatibility reaction in Prunus
    Omori*, M. Studies on the establishment of transformation system for 'Blue Muffin', a continuously flowering blueberry cultivar
  • International Botanical Congress (Jul. 23-29, Shenzhen, China)
    Invited Talk
    Akagi*, T. Evolution of flexible sex determination system in polyploid persimmon
  • IX International symposium on kiwifruit (Sep. 6th-9th, 2017, Porto, Portugal)
    Invited Talk
    Akagi*, T., H. Ohtani, T. Morimoto, K. Beppu, I. Kataoka and R. Tao. Rapid cis-evolution of a Y-encoded suppressor of feminization derived the dioecious sex determination in kiwifruits
  • Thailand Society for Horticultural Science (2017.11.30, Phisanulok, Thailand)
    Incited Talk
    Tao*, R. Molecular basis of gametophytic self-incompatibility in Prunus
    The 3rd KU-KUGSA Bilateral Symposium on “Food, Environment and Life for the Next Generation: Sustainable Development Goals” (December 4 – 5, 2017, Bangkok, Thailand)
    Oral Presentation
    Tao*, R. Self-incompatibility in Prunus
    Poster Presentation
    Malkimoto*, R. Studies on transformation of D. lotus and D. oleifera with OGI, the male determinant gene in Diospyros

2016(International)

  • Plant and Animal Genome Conference XXIV (Jan. 9-13th, 2016, San Diego, USA)
    Oral presentation
    Akagi*, T., I. M. Henry, R. Tao and L. Comai. Evolution of a flexible sex determination system in polyploid persimmon.
    Henry*, I. M., T. Akagi, R. Tao and L. Comai. Variation on a theme: diploid and hexaploid persimmon regulate sex in slightly different ways.
    Poster presentation
    Nishiyama*, S., A. Kono, N. Onoue, A. Sato, K. Ushijima, H. Yamane, R. Tao and K. Yonemori. Comparative genomics of the locus controlling astringency in Diospyros species.
  • II ISHS International Workshop on Floral Biology and S-Incompatibility in Fruit Species (May 23-26th, 2016, Murcia, Spain)
    Plenary lecture
    Tao*, R. A review of the current progress in our studies on sex determination in Diospyros.
    Oral presentation
    Akagi, T., H. Ohtani*, T. Morimoto, I. Kataoka and R. TaoUtilization of next‐generation sequencing technologies towards the identification of sex determinant in Actinidia.
    Morimoto*, T. and R. Tao. Functional characterization of Prunus SLFLs in transgenic Petunia. (This presentation was awarded the ISHS Student Presentation Award)
  • The 8th International Rosaceae Genomics Conference (June 21-24th, 2016, Angers, France)
    Oral Presentation
    Morimoto*, T., A. Wünsch, M. Watanabe, T. Akagi, and R. Tao. Next generation sequencing analysis to identify modifier gene candidates conferring pollen-part self-compatibility in sweet cherry ‘Cristobalina’.
  • The NTU- GSAKU Joint Horticulture Workshop with Students (Sept. 5-6, 2016, Kyoto, Japan)
    Oral Presentation
    Kuramoto*, A. Genetic analysis of the astringency loss in persimmon fruit
    Matsushita*, M. Functional studies on apple FLC-like involved in the regulation of chilling requirement for bud dormancy release
    Yang*, Ho-Wen. Study on the sexuality variations in oriental persimmon
  • The Second Asian Horticultural Congress (Sept. 26-28, 2016, Chengdu, China)
    Oral presentation
    Iwasa*, H., H. Yamane, M. Takai, A. Oikawa, and R. Tao. The possible invovement of abcisic acid metabolism in endodormancy release in Diospyros spp.
    Kitamura*, Y., H. Yamane, M. Gao-Takai, and R. Tao. Changes in plant hormone contents in flower buds of Japanese apricot during prolonged chilling exposure
    Naito* I., S. Nishiyama, M. Gao-Takai, H. Yamane, and R. Tao. Alteration in cytokinin metabolism is involved in small fruit mutation in Japanese persimmon (Diospyros kaki Thunb.).
    Tao*, R. Current progress in the studies on sex determination in Diospyros.
    Yamane*, T., T. Takeuchi, K. Banno, and R. Tao. Transcriptome analysis of apple (Malus x domestica) dormant buds during dormancy release.
    Poster presentation
    Yamane* H., S. Mimura, and R. Tao. Precocious flowering of Citrus seedlings and expression analysis FT/TFL1 family genes and miR156/172 involved in vegetative phase transition.
  • The ISHS I International Apple Symposium (Oct. 10-16, 2016, Yangling, Shaanxi, China)
    Oral presentation
    Yamane*, H., T. Takeuchi, M. Matsushita, K. Banno, and R. Tao. Expression analysis of apple MADS-box genes in 'Fuji' dormant flower buds.
  • The ISHS VI International Symposium on Persimmon (Oct. 16-20, 2016, Valencia, Spain)
    Plenary lecture
    Tao*, R. Sex determination in Diospyros
    Oral presentation
    Akagi*, T., L. Comai, R. Tao, and I. M. Henry. Whole genome sequencing project in persimmons
    Nishiyama*, S., N. Onooue, A. Kono, A. Sato, K. Yonemori, and R. Tao. Transcriptome analysis for astringency in persimmon fruit
    Yonemori*, K., S. Nishiyama, R. Tao, A. Kono, N. Onoue, and A. Sato. Differential expression analysis of the genes conferring non-astringent trait at the two different loci in Japanese- and Chinese-type pollination constant non-astringent (PCNA) persimmon
    Poster presentation
    Tao*, R. and T. Akagi. Possible applications of the current knowledge of sex determination in Diospyros to persimmon breeding and culture.
  • The Forum of Agricultural Meteorology, The Prevention and Utilization of the Low Temperature on Agricultural Crops (Oct. 28, 2016, Tainan, Taiwan)
    Invited Plenary Lecture
    Yamane*, H. Precedures and facilities preventing low temperature damages in Japan's orchards
  • Kyoto-Swiss Symposium2016 (Oct. 31-Nov. 1, 2016, Kyoto, Japan)
    Invited Oral Presentation
    Akagi*, T. Evolution of a flexible sex determination system in polyploid persimmon
  • Zhejiang University-Kyoto University International Symposium on Energy and Agricultural Science (Nov. 5-7, 2016, Hangzhou, China)
    Invited Oral Presentation
    Tao*, R. Sex expression in persimmons
  • The second Kasetsert University and the Graduate School of Agriculture, Kyoto University (KU-GSAKU) Bilateral Symposium on Food, Environment, and Life for the Next Generation (Dec. 6th, 2016, Kyoto, Japan)
    Oral presentation
    Yamane*, H. Introduction of the Division Agronomy and Horticultural Science, the Graduate School of Agriculture, Kyoto University and the current research highlights (Studies on genetic and molecular mechanism of chilling requirement for bud break in Japanese apricot).

2015(International)

  • Plant and Animal Genome Conference XXIII (Jan. 10-14th, 2015, San Diego, USA)
    Oral presentation
    Akagi*, T., I. M. Henry, R. Tao and L. Comai. OGI, a Y-encoded small-RNA, acts for sex determination in Diospyros plant species.
    Kajita, K., T. Akagi, H. Yamane and R. Tao*. Sexuality of persimmons.
    Henry*, I. M., T. Akagi, R. Tao and L. Comai. Transient and stable heterologous transgene expression to investigate the mechanism of sex determination in D. lotus.
  • Interrnational symposium of correlative gene system; Establishing next-generation genetics (May 29-29th, 2015, Nara, Japan)
    Oral presentation
    Tao*, R., Sexuality in Diospyros species. 
  • XIV Eucarpia Fruit breeding and genetics symposium (Jun. 14-18th, 2015, Bologna, Italy)
    Oral presentation
    Morimoto*, T., T. Akagi and R. Tao. Evolutionaly dynamics of the specificity determinant genes for the S-RNase-based self-incompatibility in the Rosaceae.
  • International Symposium on Genetic Bases for Kiwifruit Breeding (Dec. 9th, 2015, Takamatsu, Japan)
    Oral presentation
    Akagi*, T., and  R. Tao*. Successful utilization of the next-generation sequencing technologies to identify sex determinant in persimmon: A possible application for kiwifruit study.
  • The first Kasetsert University and the Graduate School of Agriculture, Kyoto University (KU-GSAKU) Bilateral Symposium on Food, Environment, and Life for the Next Generation (Dec. 17th, 2015, Bangkok, Thailand)
    Oral presentation
    Tao*, R. Introduction of the Division Agronomy and Horticultural Science, the Graduate School of Agriculture, Kyoto University and the current research highlights.

2014(International)

  • 10th International Chinese Mei Flower Symposium (Jan. 7-9th, 2014, Kunming, China)
    Oral presentation
    Yamane*, H., M. Gao-Takai and R. Tao. An attempt to control expression of PmDAMs by Agrobacterium-mediated transformation of Prunus mume
  • 7th Rosaceae Genomics Conference (June 24-26th, 2014, Seattle, USA)
    Poster presentation
    Kawai*, T., M. Nitta, A. Gonoi and R. Tao. Development of ALSV-mediated VIGS in Prunus fruit trees.
    Matsumoto*, D. and R. Tao. Formation of the SCF complex with the F-box proteins encoded by genes linked to the S locus in Prunus.
    Tao*, R., M. Watanabe and T. Akagi. Phylogenetic analysis of F-box genes homologous to the pollen S determinant F-box genes in the S-RNase-based self-incompatibility system.
  • XXIX International Horticultural Congress (August 17th-22nd, 2014, Brisbane, Australia)
    Convener
    Tao*, R. Impact of Asia-Pacific Horticulture.

2013(International)

  • Plant and Animal Genome Conference XXI (Jan. 12-16th, 2013, San Diego, USA)
    Poster presentation
    Kawai*, T. and R. Tao. ALSV-mediated VIGS in apricot (Prunus armeniaca)
  • IX International Symposium on Temperate Zone Fruits in the Tropics and Subtropics (Mar. 26-28th, 2013)
    Oral presentation
    Yamane*, H. M. Yokoyama and R. Tao. KODA slightly enhanced dormancy breaking effects of cyanamide on peach (Prunus persica) leaf buds.
    Tao*, R. Sex expression in Diospyros
  • VII International Cherry Symposium (Jun. 23-27th, 2013, Plasencia, Spain)
    Oral presentation
    Tao*, R. Molecular basis of self-(in)compatibility in Prunus fruit tree species.
    Gonoi*, A., T. Kawai and R. Tao. ALSV-mediated VIGS in fruit tree species in Prunus.
    Poster presentation
    Watanabe*, M. and R. Tao. Phylogenetic analysis of the S locus F-box genes.
  • XII International Symposium on Plant Bioregulators in Fruit Production (Jul. 28-Aug. 1st, 2013, Orlando, USA)
    Poster presentation
    Nishiyama*, S. and K. Yonemori. Effect of ABA treatment on tannin accumulation at an early stage of fruit development in persimmon (Diospyros kaki Thunb.)
    Yonemori*, K. and S. Nishiyama. Identification of plant hormones in endosperm liquid of mangosteen fruits at young developmental stages.
  • 5th Plant Dormancy Symposium (Nov. 4-7th, 2013, Auckland, New Zealand)
    Plenary speaker (invited)
    Yamane*, H. Transgenic studies of DORMANCY-ASSOCIATED MADS-box genes.

2012(International)

  • Plant and Animal Genome Conference XX (Jan. 14-18th, 2012, San Diego, USA)
    Oral presentation
    Yamane*, H., R. Sasaki, T. Habu and R. Tao. Functional studies of Japanese apricot DAM genes
    Poster presentation
    Kawai*, T., E. Nagayama, T. Akagi, H. Yamane and R. Tao. Development of the ALSV-mediated gene evaluation system for Prunus
    Habu, T., H. Yamane*, R. Sasaki, N. Yamamoto, A. Suzuki, K. Yano, H. Fujii, T. Shimizu, T. Yamamoto and R. Tao. Custom oligo DNA microarray analysis for transcript profiling of dormant buds of Japanese apricot (Prunus mume)
  • 6th International Rosaceae Genomics Conference (Sep. 30 - Oct 4th, 2012, Mezzocorona-Palarotari, Italy)
    Poster presentation
    Matsumoto*, D., H. Yamane, K. Abe and R. Tao. Identification of a Skp1-like protein interacting with SFB, the pollen S determinant of GSI in Prunus.
  • V International Symposium on Persimmon (Oct. 22-26th, 2012, Wuhan, Hubei, China)
    Oral presentation
    Tao*, R. and H. Yamane. Small-fruit mutant of ‘Hiratanenashi’ persimmon (Diospyros kaki Thunb.)
    Poster presentation
    Kajita*, K., R. Tao, K. Yonemori and H. Yamane. Phenological control of flower bud development in Diospyros kaki and D. lotus.

2011(International)

  • Plant and Animal Genome Conference XIX (Jan. 15-20th, 2011, San Diego, USA)
    Poster presentation
    Akagi*, T., T. Tsujimoto, A. Sato, A. Kono, R. Tao and K. Yonemori. Diploid/hexaploid syntenic shuttle mapping for the fruit-specific proanthocyanidin regulating gene (ASTRINGENCY gene) in persimmon (Diospyros kaki Thunb.)
  • International workshop “Floral biology and S-incompatibility in fruit species” (June 22-25th, 2011, Trento and Bologna, Italy)
    Oral presentation
    Tao, R.* S locus mutation and self-compatibility in stone fruits.
    Poster presentation
    Matsumoto, D.* and R. Tao. Isolation of a pollen-expressed actin homologous protein as a possible interactor with S-RNase in Prunus avium.

2010(International)

  • Plant and Animal Genome Conference XVIII (Jan. 9-13th, 2010, San Diego, USA)
    Poster presentation
    Jotatsu*, H., T. Ooka, R. Sasaki, H. Yamane, R. Tao and K. Yonemori. Low temperature –induced down-regulation of the MADS-box genes in endodormant buds of Japanese apricot (Prunus mume).
  • XXVIII International Horticultural Congress (Aug. 22-27th, 2010, Lisbon, Portugal)
    Poster presentation
    Yamane, H., R. Tao*, H. Jotatsu, R. Sasaki, K. Yonemori and T. Habu. EST analysis of dormant buds of Japanese apricot by massively parallel pyrosequencing.
  • 5th International Rosaceae Genomics Conference (Nov. 14-17th 2010, Stellenbosch, South Africa)
    Poster presentation
    Akagi*, T., E. Nagayama, T. Kawai, H. Yamane and R. Tao. Development of a gene evaluation system with virus-induced gene silencing in Prunus.
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